Skip to main content

Table 4 Antibacterial activities of leaf and stem extracts of R. punjabensisin terms of zone of inhibition (ZOI) and their MICs

From: Appraisal of phytochemical and in vitro biological attributes of an unexplored folklore: Rhus Punjabensis Stewart

Extracts Zone of inhibition (mm) at 100 μg/disc and MIC (μg/mL)
P. aeruginosa MIC S. typhimurium MIC M. luteus MIC B. bronchiseptica MIC S. aureus MIC
Leaf
 n-hex -- -- -- -- -- -- 13 ± 1.24*** 33.3c 12 ± 1.17* 33.3c
 CHCl3 -- -- -- -- -- -- 08 ± 0.49 -- 11 ± 1.16 100
 Acet 10 ± 1.15 100 13 ± 1.29* 33.3 18 ± 1.85*** 11.11ab 14 ± 1.23** 33.3c 08 ± 0.66 --
 EtOAc 10 ± 1.14 100 22 ± 2.25*** 1.11a 14 ± 1.12 11.11ab 11 ± 1.19 100 12 ± 1.26 100
 EtOH 06 ± 0.56 -- 10 ± 1.19 100 13 ± 1.56 33.3c 12 ± 1.11* 100 11 ± 1.18 100
 MeOH 14 ± 1.66* 33.3ab -- -- --   06 ± 0.25 -- 18 ± 19.6 11.11ab
 EtOH+ CHCl3 10 ± 1.11 100 10 ± 1.18 100 18 ± 1.65*** 11.11ab 16 ± 1.59** 33.3c 08 ± 0.77 --
 MeOH+ CHCl3 -- -- 13 ± 1.55 33.3c 10 ± 1.13 33.3c 09 ± 0.98 -- 09 ± 0.85 --
 EtOH + EtOAc 12 ± 1.55 33.3ab 18 ± 2.98*** 11.3ab 15 ± 1.44** 11.11ab 10 ± 1.12 100 13 ± 1.26** 33.3
 MeOH + EtOAc 13 ± 1.65* 33.3ab 15 ± 1.12** 11.11cb 15 ± 1.73** 11.11ab 10 ± 1.12 100 08 ± 0.66 --
 Acet + EtOAc 15 ± 1.84** 11.11a 14 ± 1.51* 11.11cb 20 ± 2.93*** 1.11a 11 ± 1.11 100 13 ± 1.56 33.3c
Stem
 n-hex -- -- 13 ± 1.37 100 10 ± 1.17 -- 12 ± 1.24 100 14 ± 1.97** 33.3c
 CHCl3 05 ± 0.78 -- 10 ± 1.14 100 -- -- 11 ± 1.14 -- 06 ± 0.57 --
 Acet 11 ± 1.27 100 15 ± 1.58** 33.3 11 ± 1.15 100 13 ± 1.67** 33.3c 10 ± 1.16 --
 EtOAc 11 ± 1.26 100 11 ± 1.71 100 14 ± 1.29** 33.3c 13 ± 1.68 -- 10 ± 1.17 --
 EtOH 10 ± 1.17 100 10 ± 1.17 100 10 ± 1.15 -- 13 ± 1.74 -- 15 ± 1.53** 33.3c
 MeOH -- -- 07 ± 0.88 -- -- -- 10 ± 1.14 -- 12 ± 1.25 --
 EtOH+ CHCl3 -- -- 07 ± 0.79 -- 12 ± 1.19 100 12 ± 1.12 -- 10 ± 1.17 --
 MeOH+ CHCl3 16 ± 1.97 33.3ab 07 ± 0.89 -- 16 ± 1.67** 11.1ab 08 ± 0.41 -- 11 ± 1.27 --
 EtOH + EtOAc 13 ± 1.10** 33.3ab 12 ± 1.10 100 11 ± 1.27   15.5 ± 1.97** 11.11ab 11 ± 1.21 --
 MeOH + EtOAc 08 ± 0.98   10 ± 1.55   12 ± 1.37 100 09 ± 0.77 -- 08 ± 0.89 --
 Acet + EtOAc 11 ± 1.81 100 12 ± 1.73 100 11 ± 1.21 -- 10 ± 1.18 -- 08 ± 0.88 --
 Cefixime 26 ± 2.98 1.1 25 ± 2.93 1.1 24 ± 2.71 1.1 24 ± 2.92 1.1 23.5 ± 2.71 1.1
 Roxithromycin 26.5 ± 2.95 0.1 25 ± 2.53 0.1 26.5 ± 2.84 0.1 26 ± 2.85 0.1 25 ± 2.32 0.1
  1. Values (mean ± SD) are average of three samples of each plant extract, analyzed individually in triplicate (n = 1x3). Means difference is highly significant*** slightly significant**, and significant* at p < 0.05. The MIC values within the column are statistically analyzed using LSD test at p <0.05. The same alphabates represent non-significant difference -- = No activity in antibacterial assay or not active (zone ˂ 10 mm) for MIC determination