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Table 3 Cytotoxic, antileishmanial and protein kinase inhibitory activities of R. punjabensis leaf and stem extracts

From: Appraisal of phytochemical and in vitro biological attributes of an unexplored folklore: Rhus Punjabensis Stewart

Samples Brine shrimp cytotoxicity
(μg/ml)
Cytotoxicity against leishmanial promastigotes (μg/ml) THP-1 cytotoxicity (μg/ml) Protein kinase inhibition (μg/disc)
  % Mortality LC50 % Mortality IC50 (μg/mL) % Mortality IC50 *Diameter (mm) at 100 μg/disc
  200   100   10   Clear zone Bald zone
Leaf
 n-hex 100 ± 4.10 36.7 ± 0.56 98.2 ± 3.12 15.78 ± 0.77 -- -- 7 ± 0.51 --
 CHCl3 98 ± 3.11 38.5 ± 0.65 90.7 ± 3.05 18 ± 0.18 64.2 ± 0.71 7.8*** ± 0.61 6 ± 0.62 --
 Acet 96 ± 3.00 52.2 ± 0.66 95.3 ± 3.18 21.60 ± 0.25*** 26.1 ± 0.10 >10 __ 9 ± 0.7
 Acet + EtOAc 92 ± 2.75 58.7 ± 0.71*** 75 ± 1.19 35.43 ± 0.41 61.9 ± 0.71 8.1** ± 0.71 8 ± 0.73 --
 EtOAc 86 ± 1.31 64.5 ± 0.78 72 ± 1.22 64.50 ± 0.62** -- -- -- 6 ± 0.56
 EtOH 82 ± 1.16 62.7 ± 0.81** 66.4 ± 0.75 75.40 ± 1.17 -- -- -- 12.5 ± 1.71***
 MeOH 65 ± 0.75 86.2 ± 1.18e 63.6 ± 0.66 81.60 ± 1.12 40.4 ± 0.61 >10 -- 13.5 ± 1.11***
 MeOH+ CHCl3 67 ± 0.71 88.1 ± 1.51 69.3 ± 0.80 77.75 ± 1.23* 35.7 ± 0.21 >10 -- 11 ± 0.87**
 EtOH+ CHCl3 62 ± 0.72 87.9 ± 1.30 54.6 ± 0.57 97.8 ± 3.11* -- -- -- 11 ± 0.88**
 EtOH + EtOAc 68 ± 0.81 82.3 ± 1.11* 68.2 ± 0.77 57.41 ± 0.75 -- -- 6.5 ± 0.11 --
 MeOH + EtOAcet 57 ± 0.55 93.2 ± 1.75 72.7 ± 1.01 54.55 ± 0.71 -- -- 6.6 ± 0.12 --
Stem
 n-hex 100 ± 4.13 39.6 ± 0.65 62.6 ± 0.80 75.74 ± 1.10** -- -- 8 ± 0.75 --
 CHCl3 98 ± 2.16 43.9 ± 0.56b 52.8 ± 0.56 78.72 ± 1.19 54.7 ± 0.71 8.4** ± 0.72 9.5 ± 0.98 --
 Acet 87 ± 1.33 55.1 ± 0.60* 48.1 ± 0.46 >100 52.3 ± 0.70 8.9* ± 0.88 6.5 ± 0.65 --
 Acet + EtOAc 76 ± 1.18 62.7 ± 0.77*** 46.6 ± 0.41 >100 -- -- 6.5 ± 0.66 --
 EtOAc 78 ± 1.19 59.5 ± 0.56** 49.2 ± 0.51 >100 -- -- 8 ± 0.85 --
 EtOH 72 ± 1.15 64.4 ± 0.68 56.7 ± 0.80 97.73 ± 3.15*** -- -- 7.5 ± 0.62 --
 MeOH 75 ± 1.17 68.7 ± 0.71** 52.2 ± 0.90 95.58 ± 1.10 -- -- -- 12.5 ± 1.12***
 MeOH+ CHCl3 62 ± 0.72 68.3 ± 0.72 28.4 ± 0.07 >100 16.6 ± 0.11 >10 -- 8 ± 0.66
 EtOH+ CHCl3 45 ± 0.09 >200 26.7 ± 0.05 >100 -- -- -- 10 ± 0.75**
 EtOH + EtOAc 42 ± 0.07 >200 18.7 ± 0.08 >100 -- -- -- 9 ± 0.96
 MeOH + EtOAc 48 ± 0.07 >200 14.4 ± 0.07 >100 -- -- 7 ± 0.70 --
 Doxorubicin 100 ± 4.10 5.75 ± 0.07       
 Flourouracil      99.6 ± 4.19 5.1 ± 0.11   
 Vincristine      98.1 ± 3.17 8.1 ± 0.92   
 Surfactin         17 ± 1.02
 Amphotercin-B    100 ± 4.01 1.36 ± 0.11   -- -- --
 1% DMSO in PBS/sea water --   --   --    
 DMSO         --
  1. *Zone of inhibition including the diameter of disc (5 mm). Data was analyzed individually in triplicate (n = 1x3). Data is presented as highly significant***, slightly significant**, significant* at p <0.05. --: no activity. LSD and HSD applied in all the data