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Table 1 Experimental design of B. monnieri effect on normal and I/R hearts

From: Bacopa monnieri extract increases rat coronary flow and protects against myocardial ischemia/reperfusion injury

Group Treatment & Protocol
Normal hearts 1) KHB (control, n = 9)
2) 30 μg/ml B. monnieri extract (n = 10)
3) 100 μg/ml B. monnieri extract (n = 8)
Protocol: Isolated hearts were perfused with KHB alone or KHB containing B. monnieri extract for 30 min (Fig. 1).
I/R hearts 1) KHB (control, n = 9)
2) 30 μg/ml B. monnieri extract (n = 9)
3) 100 μg/ml B. monnieri extract (n = 9)
Protocol: Isolated hearts were perfused with KHB alone or KHB containing B. monnieri extract for 10 min and then perfusion stopped for 30 min to emulate global myocardial ischemia, after which 30 min perfusion was resumed with normal oxygenated KHB (Fig. 2).