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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Vaccaria hypaphorine alleviates lipopolysaccharide-induced inflammation via inactivation of NFκB and ERK pathways in Raw 264.7 cells

Fig. 5

Effects of different doses of Vaccaria hypaphorine (12.5, 25 and 50 μM for 24 h) on the protein expressions of NFκB、IκBα、IKKβ and their phosphorylated protein in response to LPS-stimulated RAW264.7 cells in vitro. a, representative images of Western blot showing effects of pretreatment of different concentrations of Vaccaria hypaphorine (12.5, 25 and 50 μM), Dex (100 μM) and Asp (1 mM) on NFκB, IκBα, IKKβ and their phosphorylated protein levels of RAW264.7 cells response to LPS (1 μg/ml). b, quantitative analysis of phosphorylation of IκBα. c, quantitative analysis of phosphorylation of IKKβ. d, quantitative analysis of phosphorylation of NF-κB. Values are mean ± S.D. *P < 0.05 vs. Control, †P < 0.05 vs. LPS, †† P < 0.05 vs. Vaccaria hypaphorine (12.5 μM) + LPS. n = 6 for each group. LPS, lipopolysaccharide; Dex, dexamethasone; Asp, aspirin

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