Fig. 3

Effects of different doses of Vaccaria hypaphorine (12.5, 25 and 50 μM for 24 h) on the protein expressions of COX-2 or iNOS in response to LPS-stimulated RAW264.7 cells in vitro. a, representative images showing effects of pretreatment of different concentrations of Vaccaria hypaphorine (12.5, 25 and 50 μM), Dex (100 μM) and Asp (1 mM) on COX-2 or iNOS levels of RAW264.7 cells response to LPS (1 μg/ml). b, quantitative analysis of COX-2 or iNOS protein expressions in different groups. Values are mean ± S.D. *P < 0.05 vs. Control, †P < 0.05 vs. LPS, †† P < 0.05 vs. Vaccaria hypaphorine (12.5 μM) + LPS. n = 6 for each group. NO, nitric oxide; LPS, lipopolysaccharide; Dex, dexamethasone; Asp, aspirin. iNOS, induced nitric oxide (NO); COX-2, cyclooxygenase-2