Fig. 2

Effect of the EETC on DPB-induced PGE2 and COX-2. a The effect of DPB on PGE2 levels in various cell types. Data are expressed as mean ± SE of three independent experiments. *P < 0.01 vs. bacteria-free control medium from each cell line. b The effect of LPS extracted from DPBs (DPB^#1-LPS, DPB^#2-LPS, and DPB^#3-LPS) on PGE2 levels. PBS was used for control. *P < 0.01, ^# P < 0.001 vs. PBS control medium from each cell line. c The effect of DPBs-LPS on COX-2 expression. mRNA expression were detected by RT-PCR. The inhibitory effects of EETC on PGE2 (d) and COX-2 (e) levels in DPB-LPS-treated cells. DMSO was used for control. Data represent the mean ± SE of three independent experiments performed by triplicate. ^# P < 0.001 vs. DMSO control (Ctl) medium, *P < 0.01 vs. DPB^#1 or DPB^#1-LPS medium. f The effect of EETC on the cell viability