Fig. 1
Effect of extracts from the stems of J. multifida on the viability of MDCK cells infected with influenza A H1N1 virus. H2O, EtOAc, Hex, and CHCl3 extracts from the stems of J. multifida (0.8-25 μg/mL in DMSO) were mixed with or without (virus-) influenza A H1N1 virus (A/PR/8/34) (MOI; 10), and added to MDCK cells. DMSO (0.008-0.5%) and (+)-(S)-bakuchiol (bakuchiol; 0.8-25 μM in DMSO) were used as negative and positive controls, respectively, for the inhibition of influenza A viral infection. After incubation for 4 days, cell viability was determined by naphthol blue black staining. Data are representative of three independent experiments, and the results were found to be reproducible