Fig. 2

Effects of CS on cell-cycle distribution and apoptosis in A549 and CL1-5 cells. a Cell-cycle analysis of CS-treated cells. Cells were treated with the indicated concentrations of CS for 24 h and then subjected to cell cycle analysis. b Flow cytometry analysis of CS-induced apoptosis in A549 and CL1-5 cells. The cells were treated with the indicated concentrations of CS for 24 h and then subjected to Annexin V/PI staining. The means ± SD of the experimental triplicates are presented in the bar graph. All data are representative of three independent experiments with similar results. *P value <0.05, **P value <0.01, ***P value <0.001 compared with the control group