Exploration of anti-cancer effects and mechanisms of Zuo-Jin-Wan and its alkaloid components in vitro and in orthotopic HepG2 xenograft immunocompetent mice

Table 1 Verification of gene expression by qPCR

Gene	Sample	Average C_T	Average C_T of GAPDH	ΔC_T ^a	ΔΔC_T ^b	Fold change
EIF4A2	Mock	20.08 ± 0.26	32.36 ± 0.25	−12.28 ± 0.26	0 ± 0.26	1
	ZJW	22.97 ± 0.13	35.17 ± 0.1	−12.2 ± 0.13	0.08 ± 0.13	−1.05
	CC	22.78 ± 0.11	34.39 ± 0.09	−11.61 ± 0.11	0.67 ± 0.11	−1.59
	ER	20.79 ± 0.15	30.88 ± 0.05	−10.09 ± 0.15	2.19 ± 0.15	−4.55
	Berberine	19.32 ± 0.11	30.25 ± 0.1	−10.93 ± 0.11	1.35 ± 0.11	−2.56
	Evodiamine	19.84 ± 0.02	30.94 ± 0.02	−11.1 ± 0.02	1.18 ± 0.02	−2.27

^aThe ΔC_T value is determined by subtracting the average C_T value of GAPDH gene from the average C_T value of target gene. The standard deviation of the difference is calculated from the standard deviations of the target gene and GAPDH gene
^bThe ΔΔC_T value is determined by subtracting the ΔC_T value of mock group from the ΔC_T value of treatment group. This is a subtraction of an arbitrary constant, so the standard deviation of ΔΔCT is the same as the standard deviation of ΔC_T value
^cFold change is presented as 2^-ΔΔCT if the value is ≥ 1, or as −1/2^-ΔΔCT if the value is <1

ISSN: 2662-7671