Fig. 4

ADR (200 mg/kg) reduced infiltration of mast cells, inflammatory cells and CD4+ cells into skin. The skin sections were stained with toluidine blue (a). Sections were evaluated using microscope at an original magnification of 200x. The skin sections were stained with hematoxylin and eosin (c). Sections were evaluated using microscope at an original magnification of 200x. The skin sections were immunostained with CD4+ antibody. CD4+ cells were shown as brown color (e). b, d, f Sections were evaluated and graphed from the results of (a), (c), (e). Sections were evaluated using microscope at an original magnifiation of 1000x. Data are presented as mean ± SEM. ∗P< 0.05, ∗∗P< 0.01, and ∗∗∗ < 0.001