Fig. 3

Distribution of CGRP immune-positive fibers in extravasated dots, beside EB, and in saline control. Representative immnuofluoresence staining images of CGRP in each group was shown a and b. The corresponding images below were enlargements of the boxes outlined in a and b. CGRP (+) fibers were expressed mainly in the epidermis and dermis (white boxes). a Few CGRP (+) fibers in dermis of skin in saline-control (white box). b Distributions of CGRP (+) fibers in extravasated EB dots (b2) and beside EB dots (b1). Note that the CGRP (+) nerve fibers in EB dot were more highly expressed than that in BEB and the control (white arrow). The box in b2 show the CGRP (+) nerve fibers in the EB dot. The right corner magnification box display CGRP (+) fibers that formed a neural net around the root of the hair follicle (Scale bar: 20 μm). c, d. Sum fluorescent intensity and sum length (μm) of CGRP (+) nerve fibers per section were significantly higher in EB than in the control and in BEB (*P < 0.05 vs Control; ** P < 0.01 vs Control; # P < 0.05 vs BEB; ## P < 0.01 vs BEB)