Fig. 4

PTS increases microvascular density in ischemic cortical tissue. PTS can increase CD31 and α-SMA colocalization in ischemia boundary zones. a CD31 (red) and α-SMA (green) double-immunostaining in the ischemia boundary zone (IBZ) at 7 and 14 days after surgery. Scale bar = 50 μm. b Quantitative analysis of the microvessels in the IBZ. Data are presented as the mean ± SEM. #P < 0.05 versus Sham group; *P < 0.05 versus Veh group. n = 6 per group. c The typical detected areas of CD31 and α-SMA colocalization in boxed zones of brain slices