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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Plantamajoside from Plantago asiatica modulates human umbilical vein endothelial cell dysfunction by glyceraldehyde-induced AGEs via MAPK/NF-κB

Fig. 5

PM regulation of glycer-AGE-mediated NF-κB activation. HUVECs were co-treated with PM (10 μM) and NAC (1000 μM) including glycer-AGEs (100 μg/mL) for 4 h. NF-κB activation was detected by Western blotting or immunofluorescence staining, and the relative changes in mRNA bands were measured by Image J software. a After co-treatments, cells were collected with PBS for nucleic fraction and analyzed with 10% SDS-PAGE with GAPDH and PCNA as a control against the p65 antibody. b Total cell lysates were obtained by RIPA that contained protease inhibitors, and a phosphatase inhibitor cocktail, and then separated by 10% SDS-PAGE with GAPDH as a control. c Immunofluorescence staining shows the translocation of NF-κB to the nucleus. After co-treatments, the cells were fixed, permeabilized, and then incubated with anti-p65 antibody overnight. The cell nuclei were stained with DAPI (500 ng/mL) for 5 min and visualized using confocal microscopy

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