Fig. 3

Treatment with CS extract following the induction of CHS reaction also significantly inhibited the inflammation. Different Groups of mice (≈5 mice per group) were sensitized on the shaved ventral abdomen on days 0 and 1 by applying 50 μl of 0.5% DNFB (positive control ■), or with the vehicule alone (negative control ●) and were all challenged with 20 μl of 0.2% DNFB on the right ears on day 6. Other groups were sensitized and challenged with DNFB and received i.p. injections of CS extract on days -1, 0, 1, 2, 5, 6 and 7(panel a, CS); or on days 7, 8 and 9, following the induction of CHS reaction (panel A, CS.T) (▼). In other experiments (panel b), mice received i.p. injections of CS extract (▲) or Indomethacin (▼) at a dose of 1.07 g/Kg and 2 mg/Kg respectively for three consecutive days following the challenge (24 h, 48 h and 72 h). Data were represented as averages of ear swelling values after the challenge. P value <0.05 was considered to be significant [* p < 0.05; ** p < 0.01; *** p < 0.001] (Additional file 3)