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Table 3 Antinociceptive effect of D. sissoo leave extract, morphine and reversal effect of naloxone in tail immersion test

From: Antinociceptive effect of methanol extract of Dalbergia sissoo leaves in mice

Treatment Dose (mg/kg) Latency period (s) (% MPE) Pretreatment 30 min 60 min 90 min 120 min
Treatment without Naloxone Control 0.1 mL/mouse 2.16 ± 0.23 2.48 ± 0.16 2.68 ± 0.19 2.92 ± 0.12 3.64 ± 0.14
Morphine 5 2.03 ± 0.15 3.90 ± 0.18** (8.10) 4.90 ± 0.18*** (12.81) 5.40 ± 0.40** (14.51) 5.46 ± 0.25***(11.12)
MEDS 100 2.20 ± 0.20 3.30 ± 0.12 (4.68) 3.73 ± 0.15* (6.08) 4.37 ± 0.21 (8.50) 5.36 ± 0.24***(10.51)
MEDS 200 3.81 ± 0.18 3.72 ± 0.27* (7.10) 4.58 ± 0.45*** (10.94) 5.91 ± 0.68*** (17.49) 6.02 ± 0.14***(14.53)
MEDS 400 2.26 ± 0.09 4.02 ± 0.43** (8.80) 4.76 ± 0.23*** (12.00) 5.64 ± 0.37*** (15.92) 5.90 ± 0.18***(13.81)
Treatment with Naloxone NLX 2 1.92 ± 0.13 2.28 ± 0.16 2.77 ± 0.15 2.53 ± 0.16 2.37 ± 0.18
NLX+ Control 2 + 0.1 mL/mouse 1.77 ± 0.10 2.09 ± 0.16 2.33 ± 0.14 2.18 ± 0.11 2.63 ± 0.11
NLX+ Morphine 2 + 5 2.08 ± 0.05 2.52 ± 0.13 (0.22) 3.36 ± 0.18 (3.92) 3.98 ± 0.37 (6.20) 5.01 ± 0.30 (8.36)
NLX+ MEDS 2 + 100 2.11 ± 0.14 2.60 ± 0.11 (0.68) 3.29 ± 0.15 (3.52) 3.84 ± 0.39 (5.38) 4.82 ± 0.09 (7.21)
NLX+ MEDS 2 + 200 3.59 ± 1.14 2.63 ± 0.18 (0.85) 3.52 ± 0.29 (4.84) 3.82 ± 0.37 (5.24) 5.02 ± 0.13 (8.43)
NLX+ MEDS 2 + 400 2.04 ± 0.07 2.68 ± 0.27 (1.14) 3.56 ± 0.34 (5.09) 4.21 ± 0.25 (7.56) 5.16 ± 0.16 (9.29)
  1. Values are presented as mean ± SEM (n = 5). MEDS Methanolic extract of D. sissoo, NLX Naloxone
  2. *** p < 0.001 compared with the control group (Dunnett’s test)
  3. ** p < 0.01 compared with the control group (Dunnett’s test)
  4. * p < 0.05 compared with the control group (Dunnett’s test)