Fig. 5

Sophorae Flos extract (SFE) does not inhibit RANKL-mediated activation of PLCγ2 and CREB in osteoclasts. Bone marrow macrophages were cultured with M-CSF and RANKL in presence or absence of SFE (100 μg/ml) for 4 days. Lysate (30 μg protein) was subjected to SDS-PAGE and the phosphorylation of (a) PLCγ2 and (b) CREB analyzed by western blotting. All data are representative of at least three independent experiments