Fig. 3

SJZD Serum promoted proliferation and inhibited cell death of TNBS-damaged Caco2 cells. a Caco-2 cells were treated with various concentrations of 5–20% Control Serum (CS) or SJZD Serum (SJZDS) for 24 h, the effect of SJZDS on cell viability was measured using the MTT assay. b TNBS-damaged Caco2 cells were incubated in the medium with 15% CS or 15% SJZDS for 24 h, then stained with Hoechst 33342 and observed using a fluorescence microscope. Apoptotic cells wereindicated by the arrows (×200magnification). c TNBS-damaged Caco2 cells were treated with 15% CS or 15% SJZDS for 24 h. The induction of apoptosis was determined byAnnexin V-FITC/PI staining assay. d Quantification of the number of apoptotic cells. Data represented the mean ± SEM of at least three independent experiments (***P < 0.001 vs. control Caco2 cells group; ##P < 0.01 vs. TNBS-damaged Caco2 cells group)