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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Cryptotanshinone enhances the effect of Arsenic trioxide in treating liver cancer cell by inducing apoptosis through downregulating phosphorylated- STAT3 in vitro and in vivo

Fig. 4

ATO combined with CT inhibited xenograft tumor growth on nude mice. a The 4–6-week-old male nude mice were injected subcutaneously with 5 × 106 cells. When the subcutaneous tumors reached approximately 100 mm3, the mice were randomly assigned to four groups: control, ATO, CT, and CT combined with ATO. Tumors were measured twice a week by a vernier caliper and the tumor volume growth curve is shown (*P < 0.01 vs. control). b The mice were euthanized on the second day after 19 days of treatment. The excised tumors were weighed. Five mice were randomly selected from each group and photographed using a digital camera. c The tumor weight was calculated (*P < 0.01 vs. control; #P < 0.01 ATO + CT vs. ATO; P < 0.01 ATO + CT vs. CT). d The expressions of Phosphorylated- STAT3-Tyr705 and the related apoptotic proteins Bcl-2 and Bax were investigated by immunohistochemistry. Brown, positive; blue, nuclei (×400). e Immunohistochemistry results have been quantified by statistical software Image-Pro Plus6.0. Quantifiable results were indicated by mean optical density value

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