Fig. 3

ATO combined with CT inhibited both endogenous constitutive and Il-6–induced STAT3 activation in Bel-7404 cells. a Bel-7404 cells were divided into four groups (control, CT, ATO, and CT combined with ATO) and treated for 24 h, while whole-cell extracts were prepared and analyzed by western blotting using antibodies against phosphorylated-JAK2 and phosphorylated-STAT3^-Tyr705. b Cells were treated with ATO combined with CT for the indicated times and the expressions of phosphorylated-JAK2 and phosphorylated-STAT3^-Tyr705 were detected by western blotting. c Laser scanning confocal microscopy was used to investigate the effect of ATO combined with CT on phosphorylated-STAT3-Tyr705 expression a 4 h. The nuclei were stained with DAPI (blue), while phosphorylated-STAT3^-Tyr705 was stained with anti-phosphorylated-STAT3^-Tyr705 antibody followed by rhodamine-conjugated antibody (red). d ATO combined with CT reversed the interleukin-6–induced phosphorylated-STAT3^-Tyr705 upregulation (×800). e, g STAT3 direct target genes such as anti-apoptotic protein and promoting apoptosis proteins were detected by western blotting and the time-dependent relationships were detected. f, h Desnisity were measured by quantity one. The bar graphs were drawn based on the relative density