Fig. 2

ATO combined with CT induced liver cancer cell apoptosis. a Bel-7404 cells were divided into four groups (control, CT, ATO, CT combined with ATO). After 24-h treatment, the cells were stained using an Annexin V/PI binding kit and then analyzed by a FACS flow cytometer. b Cells were treated for 24 h, and whole-cell extracts were prepared and analyzed by western blot using antibodies against cleaved-caspase-3, cleaved-caspase-9, and cleaved-PARP. c The cells were treated with ATO combined with CT for the indicated times and the expressions of cleaved-caspase-3, cleaved-caspase-9, and cleaved-PARP were analyzed by western blotting. The experiments were performed in triplicate