Fig. 4

Western blot analysis of ER Stress markers in LS174T cells. a: Representative western blot. b: Cellular protein expression for GRP78 and CHOP in LS174T cells pre-treated, co-treated or post-treated with 0.125% and 0.25% BME for 6 h prior to, during or after ER stress induction using tunicamycin (10 ug/mL). The band densities were normalized to GAPDH band density. Data shown as means ± SEM (n = 2) (significance testing vs TM: *, **, ***; p < 0.05, p < 0.01 and p < 0.001 respectively)