Fig. 4

Effects of FBE and specific fractions on the phosphorylation of insulin-dependent Akt a and insulin-independent AMPK b. 3 T3-L1 preadipocytes were incubated with DMSO (0.1%) or 5 μg/ml of NBE, fractions F1, F2, F2-3 and two pure compounds CAT (3 μg/ml), CA (5 μg/ml) and induced to differentiate. The expression levels of key signalling proteins were measured after treatments by Western blot analysis; p-Akt, Akt a p-AMPK, AMPK b. Results are shown as the mean ± SEM. Significantly different compared to DMSO control *p < 0.05, **p < 0.01