Fig. 4

EL effects cytoskeletal organization. a A549 and d H460 lung cancer cells grown on glass coverslips were treated with DMSO or EL (100 μM) for 24 h. Immunocytochemistry was conducted using rhodamine-conjugated phalloidin to visualize F-actin fibers. Cells were imaged using an inverted fluorescence microscope (blue fluorescence represents DAPI stained nuclei and red fluorescence indicates rhodamine-conjugated phalloidin-stained F-actin filaments). The acquired images were converted into binary images for quantification on Image Pro Premier software (9.0). The density of F-actin filaments was determined for both b A549 and e H460 cells treated with or without EL. The average length of the F-actin filaments per cell was determined for c A549 and f H460 cells. p ≤ 0.05 was considered statistically significant when compared with untreated control