Skip to main content

Table 3 MRM chromatogram by UHPLC/MIS/MS method for each subject from a normal assay sample

From: Triptolide Induces hepatotoxicity via inhibition of CYP450s in Rat liver microsomes

component Corresponding CYP450s parent ion daughter ion Fragmentor /V Collision energy/V polarity
Caffeine(CAF) CYP1A2 195.0 137.8 100 20 ESI+
Tolbutamide(D860) CYP2C9 269.1 169.8 110 13 ESI-
(S)-Mephenytoin(MT) CYP2C19 219.0 134.0 100 17 ESI+
Dextromethorphan(DM) CYP2D6 272.0 214.9 155 25 ESI+
Chlorzoxazone(CLZ) CYP2E1 168.0 131.8 120 17 ESI-
Midazolam(MDZ) CYP3A 326.0 291.0 106 25 ESI+
Paraxanthine CYP1A2 181.0 123.9 120 21 ESI+
4-Hydroxy Tolbutamide CYP2C9 287.0 74.2 95 13 ESI+
(+/−)-4-Hydroxy Mephenytoin CYP2C19 235.0 150.0 90 17 ESI+
Dextrorphan CYP2D6 258.2 157.0 145. 40 ESI+
6-Hydroxy chlorzoxazone CYP2E1 183.9 64.1 100 33 ESI-
1-Hydroxymidazolam CYP3A 342.0 324 162 21 ESI+
Diazepam(DZP) ISTD 285.0 193.0 159 33 ESI+