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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: Triptolide Induces hepatotoxicity via inhibition of CYP450s in Rat liver microsomes

Fig. 6

Effects of TP on the CYP450s enzyme activity. a The effect of TP on the substrate metabolic rate. After orally treated with vehicle or 200, 400 or 600 μg/kg/day of triptolide for 28 days, the liver microsomes were incubated with the cocktail probe (CAF/D860/MT/DM/CLZ/MDZ: 15/20/10/10/5/2.5 μM) for 30 mins. The final concentration of the substrates and metabolites were detected by UPLC-MS/MS. the substrate metabolic rate of CYP1A2, CYP2D6 after the 30-min-incubation in vitro remained the same but other CYP450s showed significant changes. The 200 μg/kg/day TP group exhibited decreases in CYP2C19 and CYP3A; then the 400 and 600 μg/kg/day TP group exhibited decreases not only in CYP2C19 and CYP3A, but also CYP2E1 and CYP2C9. b The effect of TP on the production of the metabolites. After the administration, the 200 μg/kg/day TP group showed decreases in CYP2C19 and CYP3A, then the 400 and 600 μg/kg/day TP group also exhibited decreases in CYP2E1 and CYP2C9. Results are expressed as mean ± SD of each group, respectively;*P < 0.05, ** P < 0.01 significantly different from the control group

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