Fig. 5

The inhibitory effect of CBTE on the LPS-induced nuclear factor-kappa B protein expressions in RAW264.7 cells. The levels of I-κBα, p-I-κBα (30 min in the cytosol fraction) and NF-κB (1 h in the nuclear fraction) protein were monitored with or without CBTE pre-treatment (i.e. 1 h before LPS). β-actin and lamin A/C were used as a loading control (a and d). The relative levels of I-κBα (b) p-I-κBα (c) and NF-κB (e) were measured by scanning densitometry. Values represent the mean ± S.D. of three independent experiments (significant as compared to the control, **P < 0.01, significant as compared to LPS alone, ^## P < 0.01). CBTE, Cheongsangbangpung-tang extract; NF-κB, Nuclear factor-kappa B; I-κB, Inhibitor-kappa B