Fig. 4From: Anti-aging and tyrosinase inhibition effects of Cassia fistula flower butanolic extractThe effect of C. fistula flower extract on collagenase activity was determined using fluorogenic DQ™-gelatin assay. Various concentrations of the flower extract (0–200 μg/mL) were mixed with collagenase. After that, DQ gelatin substrate was added and incubated for 10 min. The rate of proteolysis was evaluated using a microplate reader. All assays have been demonstrated in triplicate and the mean ± standard deviations are shown as ****p < 0.0001 versus the control without the extractBack to article page