Fig. 3
From: Anti-aging and tyrosinase inhibition effects of Cassia fistula flower butanolic extract
The effects of C. fistula flower extract on collagen synthesis were determined using a collagen kit. Fibroblast cells were pre-treated in serum free medium for 24 h. and were further treated with or without various concentrations of flower extract (0–150 μg/mL) for 48 h. The cells were stained with Sirius Red/Fast Green dye and the absorbance was determined using a UV-spectrophotometer. All assays have been performed in triplicate and the mean ± standard deviations are shown as ****p < 0.0001 versus the non-treated cells