Effects of the Ayurved Siriraj Wattana recipe on functional and phenotypic characterization of cytokine-induced killer cells and dendritic cells in vitro

Table 1 Primer pair and their information for quantitative real-time PCR

Genes	Oligonucleotides (5′ → 3′)	Size (bp)	Annealing (°C)
GAPDH	Forward: GAAATCCCATCACCATCTTCC	124	60
GAPDH	Reverse: AAATGAGCCCCAGCCTTCTC	124	60
IDO	Forward: AGTCCGTGAGTTTGTCCTTTCAA	68	60
IDO	Reverse: TTTCACACAGGCGTCATAAGCT	68	60
IFNγ	Forward: GTGTGGAGACCATCAAGGAAGAC	80	60
IFNγ	Reverse: CAGCTTTTCGAAGTCATCTCGTTT	80	60
IL-4	Forward: AACAGCCTCACAGAGCAGAAGAC	101	60
IL-4	Reverse: GCCCTGCAGAAGGTTTCCTT	101	60
IL-6	Forward: GCTGCAGGCACAGAACCA	68	60
IL-6	Reverse: ACTCCTTAAAGCTGCGCAGAA	68	60
IL-10	Forward: CTGGGTTGCCAAGCCTTGT	100	60
IL-10	Reverse: AGTTCACATGCGCCTTGATG	100	60
IL-12	Forward: GCAAAACCCTGACCATCCAA	100	60
IL-12	Reverse: TGAAGCAGCAGGAGCGAAT	100	60
IL-17	Forward: ACCTGTGTCACCCCGATTGT	90	58
IL-17	Reverse: GGGTCGGCTCTCCATAGTCTAA	90	58
GATA3	Forward: ACTACGGAAACTCGGTCAGG	100	60
GATA3	Reverse: CAGGGTAGGGATCCATGAAG	100	60
STAT1	Forward: GTGGCGGAACCCAGGAAT	97	60
STAT1	Reverse: TGACAGAAGAAAACTGCCAACTCA	97	60
STAT3	Forward: ACCAAGCGAGGACTGAGCAT	90	58
STAT3	Reverse: TGTGATCTGACACCCTGAATAATTC	90	58
STAT4	Forward: TTCCTTCTGTTTTTATCCCCATCT	128	60
STAT4	Reverse: TGTTGTGGGACTCAGGTTTTCTC	128	60
STAT5A	Forward: CACGCAGGACACAGAGAATGA	80	58
STAT5A	Reverse: TCAGGCTCTCCTGGTACTGGAT	80	58
STAT5B	Forward: GGTCACGCAGGACACAGAGAA	110	58
STAT5B	Reverse: CCAGCGGGCCAAACTG	110	58
STAT6	Forward: CTTTTGGCAGTGGTTTGATGGT	96	60
STAT6	Reverse: TGTTTGCTGATGAAGCCAATG	96	60
T-bet	Forward: AGGATTCCGGGAGAACTTTGA	123	60
T-bet	Reverse: TACTGGTTGGGTAGGAGAGGAGAGTA	123	60
RORC	Forward: CCACAGAGACATCACCGAGCC	114	60
RORC	Reverse: GTGGATCCCAGATGACTTGTCC	114	60