Fig. 5
Ex vivo effects of WIB-801CE on phosphorylation of p38 MAPK and ERK2. a Ex vivo effects of WIB-801CE on collagen-induced phosphorylation of p38 MAPK. Lane 1, unstimulated platelets; Lane 2, collagen (10 μg/mL); Lane 3, collagen (10 μg/mL) + WIB-801CE (200 mg/kg-BW); Lane 4, collagen (10 μg/mL) + WIB-801CE (400 mg/kg-BW). b Ex vivo effects of WIB-801CE on ADP-induced phosphorylation of p38 MAPK. Lane 1, unstimulated platelets; Lane 2, ADP (5 μM); Lane 3, ADP (5 μM) + WIB-801CE (200 mg/kg-BW); Lane 4, ADP (5 μM) + WIB-801CE (400 mg/kg-BW). c Ex vivo effects of WIB-801CE on collagen-induced phosphorylation of ERK2. Lane 1, unstimulated platelets; Lane 2, collagen (10 μg/mL); Lane 3, collagen (10 μg/mL) + WIB-801CE (200 mg/kg-BW); Lane 4, collagen (10 μg/mL) + WIB-801CE (400 mg/kg-BW). d Ex vivo effects of WIB-801CE on ADP-induced phosphorylation of ERK2. Lane 1, unstimulated platelets; Lane 2, ADP (5 μM); Lane 3, ADP (5 μM) + WIB-801CE (200 mg/kg-BW); Lane 4, ADP (5 μM) + WIB-801CE (400 mg/kg-BW). Western blotting was performed as described in “Methods” section. The data are expressed as the mean ± standard deviation (n = 4). *p < 0.05 versus the each agonist-stimulated platelets. 1) Δ (%) = [(agonist + WIB-801CE 200 mg/kg-BW) – agonist]/agonist × 100