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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Antiplatelet and antithrombotic effects of cordycepin-enriched WIB-801CE from Cordyceps militaris ex vivo, in vivo, and in vitro

Fig. 3

Ex vivo effects of WIB-801CE on phosphorylation of cPLA2, PLCβ3, PLCγ2, release of AA, and activity of COX-1 and TXAS. a Ex vivo effects of WIB-801CE on collagen-induced phosphorylation of cPLA2, PLCβ3 and PLCγ2. Lane 1, unstimulated platelets; Lane 2, collagen (10 μg/mL); Lane 3, collagen (10 μg/mL) + WIB-801CE (200 mg/kg-BW); Lane 4, collagen (10 μg/mL) + WIB-801CE (400 mg/kg-BW). b Ex vivo effects of WIB-801CE on ADP-induced phosphorylation of cPLA2, PLCβ3 and PLCγ2. Lane 1, unstimulated platelets; Lane 2, ADP (5 μM); Lane 3, ADP (5 μM) + WIB-801CE (200 mg/kg-BW); Lane 4, ADP (5 μM) + WIB-801CE (400 mg/kg-BW). c Ex vivo effects of WIB-801CE on collagen-induced arachidonic acid release. d Ex vivo effects of WIB-801CE on ADP-induced arachidonic acid release. e Ex vivo effects of WIB-801CE on COX-1 activity f Ex vivo effects of WIB-801CE on collagen-induced TXAS activity. g Ex vivo effects of WIB-801CE on ADP-induced TXAS activity. Measurements were carried out as described in “Methods” section. The data are expressed as the mean ± standard deviation (n = 4). *p < 0.05 versus each agonist-stimulated platelets. NS, not significant versus the each agonist-stimulated platelets, #p < 0.05 versus the ADP-stimulated platelets in the presence of WIB-801CE 200 mg/kg-BW. 1) Δ (%) = [(agonist + WIB-801CE 200 mg/kg-BW) – agonist]/agonist × 100

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