Fig. 3

Activation of ERK and CREB by PSR. a Western blots and quantitative densitometry (b) showing the effect of 0–180 min of PSR 500 μg/mL treatment on ERK and CREB phosphorylation. Band intensities in western blots (n = 2) were quantified using ImageJ. The band intensity of the phosphorylated ERK/CREB protein is first normalized to that of the corresponding total ERK (or CREB) protein, and then the value of the normalized band intensity of the phosphorylated protein at time 0 was set as 1. Statistical significance in (b) was performed by one-way ANOVA followed by Newman-Keuls post-test to compare the band intensity at each time point to that of time 0. c Effect of 6 h-treatment of PSR on the luciferase activity of neurons transfected with the CRE-Luc construct. Seven concentration-response experiments, with each concentration of PSR conducted in duplicate wells, were performed. For PSR 100 to 1000 μg/mL, n = 5, 6, 7, 7, 4, respectively. Statistical significance between PSR-treated and control groups was analyzed by one-way ANOVA followed by Newman-Keuls post-test