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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Neuroprotective effect of a novel Chinese herbal decoction on cultured neurons and cerebral ischemic rats

Fig. 2

PSR protects neurons against NMDA excitotoxicity. a Rat cortical neurons were treated with vehicle (top panels), 20 μM NMDA (middle panels), or 20 μM NMDA plus PSR 500 μg/mL (bottom panels). Phase contrast images are shown on the left. The right panels show the overlay of the DAPI- and β-tubulin III-stained images. b LDH released from NMDA-challenged neurons with or without PSR treatment was measured and quantified as normalized cell death (see Methods). Four concentration-response experiments, with each concentration of PSR conducted in duplicate wells, were performed. For PSR 30, 750 and 1000 μg/mL, number of data points (n) = 2; for PSR 100, 300 and 500 μg/mL, n = 4. Statistical significance between PSR-treated and control groups was analyzed by one-way ANOVA followed by Newman-Keuls post-test. c Representative whole-cell current-response recorded from a hippocampal neuron. NMDA-induced current was reversibly inhibited by PSR 500 μg/mL. d Inhibitory effect of PSR 500 μg/mL on NMDA-induced current. The control was the NMDA-induced current level in the absence of PSR (set as 100 %). Statistical significance was analyzed by paired t-test (n = 4)

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