Fig. 3

Inhibitory effect of EA/EuH on the invasive capability of MDA-MB-231 cells and experimental lung metastasis of 4 T1 cells. a MDA-MB-231 cell spheroids in the extracellular matrix were either untreated (control) or treated with TNFα (10 ng/mL) in the absence or presence of EA/EuH (5 μg/mL). Morphology of 3-D cell invasion was captured at 3-days and 7-days with an EVOS FL Auto Cell Imaging System. b Mouse 4 T1 mammary tumor cells were implanted into the spleen of Balb/c mouse. At 1 day post intra-splenic implantation of 4 T1 cells, either phosphate buffered saline (PBS) or EA/EuH (10 mg/kg) was administered intraperitoneally daily. After 8 days, mice were sacrificed. Lung tissues were fixed and stained with hematoxylin and eosin (H&E)