Fig. 1

Protection from oxaliplatin-mediated neurotoxicity by WLR in differentiated PC12 cells. a PC12 cells were cultured on a collagen type IV-coated surface and neurite growth was initiated by the treatment of 100 ng/mL NGF. A combination of oxaliplatin (L-OHP, 200 nM) and WLR (L, 25 μg/mL; H, 100 μg/mL) were applied to the cell culture. Amifostine (Ami, 0.5 mM) and vehicle (V, PBS) were also included in parallel as positive and negative controls, respectively. Following 3 days of differentiation, the neurites sprouting from the cell bodies were observed under an inverted microscope (X100). b The percentages of cells bearing neurites and total neurite lengths were quantified using an image analyzing program. The data represent the combined results of n = 3 experiments. **p < 0.01 vs. only NGF-treated group (NGF); ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 vs. NGF, L-OHP, and vehicle-treated group (NGF + L-OHP + V)