Estrogenic and cytotoxic potentials of compounds isolated from Millettia macrophylla Benth (Fabaceae): towards a better understanding of its underlying mechanisms

Table 2 Cytotoxicity of isolates of M. macrophylla as well as phenolic fraction toward breast cancer cells

		CC50
Code	Name of compounds	MCF-7 cells	MDA-MB-231 cells
1	lupenone	>500	>500
2	lupeol	110	160
3	stigmastenone	>500	>500
4	palmitic acid	>500	>500
5	daidzein dimethylether	>500	>500
6	formononetin	>500	342
7	afromorsin	>500	224
8	secundiferol I	>500	457
9 2’	OH-formononetin	>500	>500
10	(+)-pisatin	>500	>500
11	flemichapparin B	>500	>500
12	dihydrocoumestrol dimethylether	>500	>500
13	variabilin	>500	296
PF	phenolic fraction	452	327

MCF-7 and MDA-MB-231 cells were incubated with increasing concentration (100–500 μM) of isolates of M. macrophylla and phenolic fraction -PF (100–500 μg/mL) for 24 h. Cell viability was evaluated by Alamar blue assay. The CC50 was determined when applicable by nonlinear regression analysis of the logarithm of concentration in function of the normalized response (percentage of cell viability). Values are in μM or μg/mL mean of three independent experiments. The Bold data reflect the exact cytotoxic concentration (CC₅₀)

ISSN: 2662-7671