Fig. 2

Effect of saffron extract and crocin on liver steatosis based on histopathological examination (H&E staining). Representative histopathological examination of H&E staining of liver tissue prepared from experimental rats fed with a normal diet (ND), b high-fat diet (HFD), c high-fat diet + crocin 40 mg/kg (HFD + L-CRO), d high-fat diet + crocin 80 mg/kg (HFD + H-CRO), e high-fat diet + saffron extract 40 mg/kg (HFD+ L-SAF), and f high-fat diet + saffron extract 80 mg/kg (HFD + H-SAF) (magnification 400×). Major histopathological changes induced by HFD in rat liver were hepatosteatosis, ballooning and inflammation of hepatocytes