Fig. 9

DNA fragmentation based on TUNEL assay on A549 cells treated with chalepin at various concentrations at 48 h and 72 h incubation. a R3 region in the dot plots obtained from flow cytometry represents TUNEL positive staining in which cells were stained with FITC-conjugated anti-BrdU antibody which resulted from treatment of chalepin on A549 cells at 48 h incubation. b R3 region in the dot plots obtained from flow cytometry represents TUNEL positive staining in which cells were stained with FITC-conjugated anti-BrdU antibody resulted from treatment of chalepin on A549 cells at 72 h incubation. c Bar chart represents the percentage of TUNEL positive cells. Histograms are representative of three separate experiments (n = 3). Asterisks indicate significantly different value from control (*p < 0.05)