Fig. 11
Elevation of the intracellular reactive oxygen species (ROS) level was observed qualitatively through observation of the cells after incubating with DCFH-DA dye and examining the changes using a fluorescent microscope and quantified using a fluorescence plate reader. a Induction of ROS in A549 cells treated with chalepin at various concentrations and incubated for 24 h and 48 h b Bar chart represents the fold increase of intracellular ROS level in A549 cells treated with different concentrations of chalepin as compared to untreated negative control cells. The data expressed as mean ± S.D. from three replicates. Asterisks indicates significantly different value from control (*p < 0.05)