Fig. 2

The effect on PF on the protein expression of cyclin D1 and CDK4 in human colorectal cancer cells. a-d The cells were treated with the indicated concentrations of PF for 24 h. e HCT116 cells treated with PF (25 μg/ml) for the indicated times. Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibodies against cyclin D1 and CDK4. Actin was used as internal control for Western blot analysis. *P < 0.05 compared to cell without PF treatment