Fig. 3
Effect of the HPLC fraction 10 on cell morphology, viability and activation of caspase-3. a Effect of the HPLC fraction 10 on triggering cytotoxic insult in HepG2 cancer cells after 3 h of incubation in the concentration range 0.001–10 mg/mL. Note apoptotic cells with different concentrations (see arrows). b Viability of HepG2 cells measured by FACS after treating them in the concentration range 0.001–10 mg/mL with the HPLC fraction 10. Note that for all experiment the second stable fraction eluted after 10 min was used. Effect on cells pretreated with z-VAD-fmk was measured as well. Grey bars are representing the percentage of apoptotic cells and black bars percentage of dead cells. Error bars denote mean ± SEM. *p < 0.05; ** p < 0.001; *** p < 0.0001. c DEVD-ase activity (caspase −3 activity) in HepG2 cells after the treatment of the cells with HPLC fraction 10 in the concentration range 0.001–10 mg/mL. DEVD-ase activity measured in arbitrary units (RFU). Error bars denote mean ± SEM. *p < 0.05; ** p < 0.001; *** p < 0.0001