Table 2 Sequence of designed primers for each gene is shown as forward and reverse. The primers used here for real time PCR were designed by www.GeneScript.com according their accession number
Gene | GenBank accession Number | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|---|
GAPDH | [NM_017008.3] | ATGACTCTACCCACGGCAAG | CTGGAAGATGGTGATGGGTT |
MMP-9 | [NM_031055] | TCGAAGGCGACCTCAAGTG | TTCGGTGTAGCTTTGGATCCA |
MMP3 | [NM_133523] | TCTTTCACTCAGCCAATGCT | GGGAGGTCCATAGAGGGATT |
IL-6 | [NM_012589.1] | AGTCCGGAGAGGAGACTTCA | TTGCCATTGCACAACTCTTT |
TNFα | [NM_012675.3] | CCACCACGCTCTTCTGTCTA | GCTTGGTGGTTTGCTACGA |
TGFβ1 | [NM_031131.1] | CTGAACCAAGGAGACGGAAT | GGTTCATGTCATGGATGGTG |