Evaluation of the anti-diarrheal activity of the leaf extract of Croton macrostachyus Hocsht. ex Del. (Euphorbiaceae) in mice model

Table 3 Effect of the fractions of Croton macrostachyus leaves on castor oil induced intestinal transit in mice

Dose administered	Mean length of small intestine (cm)	Mean Distance traveled by the charcoal meal (cm)	Peristalsis index (%)	% of inhibition
Control	58.75 ± 2.48	55.32 ± 2.78	94.04 ± 1.74	------
Atropine sulphate 5 mg/kg (i.p)	60.27 ± 0.46	18.92 ± 2.59^a*^c	31.33 ± 4.22^a*^c	65.78
CF300mg/kg	57.57 ± 2.38	41.22 ± 0.83^a^b*	72.25 ± 3.42^a^b*	25.49
CF400mg/kg	58.12 ± 2.18	31.33 ± 4.89^a**	54.56 ± 8.71^a**	43.36
CF500mg/kg	61.03 ± 0.945	29.00 ± 7.09^a**	47.37 ± 11.15^a**	52.42
Control	60.05 ± 2.06	56.88 ± 2.73	94.55 ± 1.78	-------
Atropine sulphate 5 mg/kg (i.p.)	63.1 ± 1.32	24.13 ± 4.24^a**^c	38.71 ± 7.45^a**^c	57.58
MF300mg/kg	61.25 ± 1.52	43.25 ± 5.45^b*	70.12 ± 8.38^b^e	23.96
MF400mg/kg	66.28 ± 2.49	42.87 ± 5.89	64.59 ± 8.36	24.63
MF500mg/kg	63.07 ± 1.88	25.00 ± 3.39^a***	39.78 ± 5.66^a**^c	56.05
Control	62.68 ± 3.54	50.65 ± 3.88	80.81 ± 4.02	-------
Atropine sulphate 5 mg/kg(i.p.)	61.05 ± 2.86	18.72 ± 3.99^a^c^d*	30.66 ± 6.26^a^c^d*	63.04
AF300mg/kg	63.55 ± 1.47	50.08 ± 2.65^b*	78.8 ± 6.04^b^f*	1.12
AF400mg/kg	63.45 ± 1.54	49.25 ± 5.76^b^f	77.62 ± 8.54^b^f*	2.76
AF500mg/kg	61.98 ± 3.69	38.23 ± 7.74^f*	61.68 ± 11.67	24.5
AF1000mg/kg	68.00 ± 1.53	27.45 ± 2.21^a^c^d*	40.53 ± 3.55^a^c^d*	44.26

Values are expressed as Mean ± S.E.M (n = 6), analysis was performed with One-Way ANOVA followed by Tukey test;^a compared to control,^b to standard drug,^c to 300 mg/kg,^d to 400 mg/kg,^e to 500 mg/kg,^f to 1000 mg/kg,^g to CF300 mg/kg,^h toCF 400 mg/kg and^I to CF500 mg/kg; *P <0.05, **P <0.01, ***P <0.001; CF chloroform fraction, MF methanol fraction, AF aqueous fraction. Negative controls were treated with the vehicle used for reconstitution (2 % v/v Tween 80 for the chloroform and methanol fractions or distilled water for the aqueous fraction) orally