Fig. 5
Effect of isolated 3-O-methyl quercetin (S3) and kaempferol (S4) on expression ofoxidative stress markers. a Fold change of Nrf-2 gene expression in lung and liver cells pre-treated with 3-O-methyl quercetin and kaempferol. The Nrf2 levels in nuclear and cytoplasmic fractions of lung and liver cells pre-treated with 3-O-methyl quercetin and kaempferol and results were expressed as percent control (b). Western blot analysis of SOD2 and catalase in lung (c) and liver (d) cells pretreated with 3-O-methyl quercetin and kaempferol (100 μg/ml) followed H2O2 (100 μM). Quantification of SOD2 and catalase expression in S3 (e) and S4 (f) treated lung cells and S3 (g) and S4 (h) treated liver cells by densitometry