Fig. 5
Cell cycle analysis of colonic epithelial cells treated with or without DPE. Colon Epithelial Cells 1x106 cells were cultured in BrdU and the cells were cultivated for 24 h. (A). The harvest cells assessed with BrdU followed by labeling with a FITC conjugated anti-BrdU antibody and Propidium Iodide for the cell cycle were analyzed as described in Materials and Methods and the percentages of S phase, G0/G1 and G2/M in the areas have shown. Data are representative of four independent experiments with similar results. (B) The percentage of cells in S phase. Results shown are mean + SD, with n = 4 replicates in each group. Results are mean ± SD. *, p < 0.05. **, p < 0.001