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Table 2 Gradient Table for Load pump

From: Isolation of alpha-linolenic acid from Sutherlandia frutescens and its inhibition of Mycobacterium tuberculosis’ shikimate kinase enzyme

Time (min)

Flow (mL/min)

%A IPA

%B MeOH

%C HOH

%D CAN

0.0

1.0

0

100

0

0

25.0

1.0

0

50

0

50

30.0

1.0

0

0

0

100

31.0

1.0

100

0

0

0

38.0

1.0

100

0

0

0

40.0

1.0

0

100

0

0

50.0

1.0

0

100

0

0