Fig. 2

CD133⁺ SFCs from NCI-H446 cell line exhibited higher self-renewal capacity compared to that of parental cells. a, The sphere-forming rate of CD133 ⁺ SFCs and parental cells (PC). b, Tumor sphere formation by single cell dissociated from CD133 ⁺ SFCs derived from SCLC NCI-H446 cell line (200 × magnification). c, The expression levels of self-renewal related transcription factors (Bmi1, Nanog and Oct4) and stem cell markers were compared between CD133 ⁺ SFCs and parental NCI-H446 cells at 48 h. d, In vitro invasion capacity were compared between CD133 ⁺ SFCs and parental NCI-H446 cells (400 × magnification). e, The expression levels of invasion related transcription factors (Twist1 and Snail1) were compared between CD133 ⁺ SFCs and parental NCI-H446 cells at 48 h