Fig. 5From: Trichloromethane fraction of Incarvillea compacta induces lytic cytotoxicity and apoptosis in Epstein-Barr virus-positive gastric cancer AGS cellsThe effects of R2 on the expressions of lytic-related mRNA in AGS-EBV (a), the expressions of lytic-related proteins in AGS-EBV (b and c) and LCL cells (Fig. 5d and Fig. 5e) were determined by RT-PCR and western blot. AGS-EBV and LCL cells were treated with R2 (0, 5, 10, 20 μg/mL) for 24 h. The mRNA expressions of BZLF1, BMRF1 and BRLF1 increased after R2 treatment. R2 also increased the protein expressions of BZLF1 and BMRF1. Relative expression levels of EBV lytic-related proteins were showed in Fig. 6c and e. β-actin was used to confirm equal mRNA and protein loading. *P < 0.05 and ** p < 0.01 were considered statistically significantBack to article page