Fig. 4

Effects of R2 on the expression of apoptosis and cell cycle related proteins in AGS-EBV cells were detected by western blot. AGS-EBV cells were treated with R2 (0, 5, 10, 20 μg/mL) for 24 h. R2 decreased the expressions of Bcl-2 and cyclin D1, while increased the expressions of Bax and Rb (a). Relative expression levels of proteins were showed in (b). β-actin was used to confirm equal protein loading. Results obtained from a representative experiment were shown (n = 3). *P < 0.05 and ** p < 0.01 were considered statistically significant