Fig. 4

The percentage of viable, apoptotic and necrotic/secondary necrotic cells of untreated andampelopsin E-treated MDA-MB-231 cells for 24 and 48 h as determined by flow cytometer. a and b These are from representative experiments carried out at least three times. The percentage of viable cells were represented by lower left quadrant (Annexin-V⁻/PI⁻); the percentage of early apoptotic and necrotic/secondary necrotic cells were represented by the lower right (Annexin-V⁺/PI⁻) and upper (PI⁺) quadrants, respectively. Each data point represents the mean of three independent experiments ± SD. *significantly different from the control (p < 0.05)